FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155423
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG1502.7645001E-838.39884
GTATAAC1951.8189894E-1036.9491041
TCTGTCG3600.033.998948
GTCTTGC11950.033.1614341
TCGCTCT5000.033.1189654
GTCTCGC5300.031.267311
GTATTAT2559.458745E-1131.0807171
TAATACT3300.030.54454
GTATTAA3052.5465852E-1128.3478371
GTATTAC2553.2323442E-928.2551981
TCTCGCT5400.028.0197352
TACACGT1555.767961E-527.8700985
GTATAGT2853.4742698E-1027.8090651
GTGTAAT4150.027.7786051
GTCTTAA3900.027.7118281
CTGTCGC5500.027.490059
GTGTTAG8700.026.5014291
GTCTATG4400.026.2002741
GTATATA4950.026.2002741
CGCTCTG6350.026.0779255
Produced by FastQC (version 0.11.5)