FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155423
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC850.002837146433.8814773
GTCTTAC4100.033.3693241
GTCTTGC9150.033.0526161
TCTGTCG6100.033.049768
GTACTAC1751.071694E-732.917711
GTATTAG3400.031.768011
GTATAAG3650.031.5649261
GTCTCGC5250.031.5461371
TACACTA1952.77043E-729.5389775
GTAATAG2553.25781E-928.2345643
TAGCGCT1358.418159E-426.6659774
GTGTTAC4600.026.6114621
TTGCTCT13550.026.5675774
TCGCTCT5000.025.9193294
TACCCGG1400.001040632725.7147355
GTATTAC2259.667492E-725.6026631
GCGCGAT3109.313226E-1025.5488329
GGATAAC3151.1186785E-925.1454731
GTCGTGA1450.001276271424.8301681
TTACACT4400.024.544824
Produced by FastQC (version 0.11.5)