FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907430
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7300.046.3548058
GTCTCGC8650.039.9829831
TCGCTCT9600.034.498994
GGTATAC4350.033.1276631
GTGTAAC4900.032.3501741
TCTCGCT10350.032.0234072
CGCTCTG10300.030.7563785
CTGTCGC10350.029.9121659
CTCGCTC10400.029.7910043
GTATAAT4900.029.4092481
GTGTTAC8250.027.94771
GTGTAGA9100.027.7125631
GTATAGA5550.027.2631721
GTATTAG6100.027.1673981
GTCTTGC17150.026.8884581
GTATTAT5050.025.6821381
GTGCTAC7050.024.5285661
GTGTAGG10450.024.1324711
GTGTTAG12050.023.9178961
TTACACT8300.023.4210014
Produced by FastQC (version 0.11.5)