FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907430
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7600.041.6879771
TCGCTCT8950.037.005054
GTCTTGC17650.036.7171971
GTATTAC3950.032.813091
GTATTAA4400.031.0937181
TCTCGCT10350.029.2169652
CGCTCTG10200.028.9415135
GTCTTAC6900.028.1764561
CTCGCTC10250.028.0971533
GGTATAC3850.028.0544821
CGGAACT1804.9635873E-627.9995944
TTGCTCT23950.027.3565944
GTGTTAC8200.027.221971
GTATAAT6200.026.7120911
GTATTAG6050.026.1841831
GTACGGA1658.822967E-526.1841811
TCTGTCG10300.025.1653428
GTACAGA10550.024.5709361
GTGTAAG9350.023.8738161
CTTGCTC25050.023.568523
Produced by FastQC (version 0.11.5)