FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124695
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.049.5317421
TCGCTCT11500.039.4432954
GTACGGA1851.03682396E-1038.925151
TCTCGCT12500.038.5918242
CTCGCTC12400.036.580483
GTCTTGC16800.033.0052871
TCTGTCG11250.032.6398548
CGCTCTG13150.032.3040355
GTGTTAC8150.031.8087771
TACGGAC1402.8812401E-530.8570022
GTACTAT3800.030.3206441
GTCTTAC8000.029.704761
TAATACT5950.026.6217274
GTGTAAG9100.026.1140731
GTATTGA5850.025.8502941
GTGTTAG13400.025.7951761
GGGTATA7000.025.7184051
GTGTAAA11500.025.6736771
GTGTATA11550.025.5625361
GTACTAG3451.1823431E-1025.0474911
Produced by FastQC (version 0.11.5)