FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391307
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.034.1504678
GTCTCGC13150.029.7060571
GTCTTGC26350.029.100621
CTGTCGC15300.028.7061089
TCGCTCT15300.027.2931254
TTCACCG8550.025.2618685
GTATTAA8400.024.9744381
TCTCGCT17350.024.0707742
CTCGCTC16550.023.928743
GTCTCAC31950.023.3207951
CGCTCTG15750.023.3130975
ACCGTGT9400.022.9789038
GTCTAGC11400.022.8441331
GTACTAT6700.022.6736531
GTGTTAC12450.022.0796031
GTGTTAA12850.021.39231
TCACCGT10100.020.672216
TTGCTCT37950.020.4893744
GGAGTGC66300.020.4152455
GTATTAG9350.020.1158661
Produced by FastQC (version 0.11.5)