FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391307
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13850.031.8742281
TCTGTCG14600.029.0915588
GTCTTGC27150.027.641941
TCGCTCT15950.026.6296564
CTCGCTC15950.024.8242553
GTACCGA1500.001557831423.9964286
GTGTAAG14200.022.7983041
GTATTAC7850.022.4946771
GTGTTAC11600.022.1997591
TCTCGCT18550.021.7438342
CGCTCTG17650.021.6171765
GAGTGCA70300.021.6070256
GGAGTGC63850.021.4220285
GTCTTAC10400.021.2239441
GTGTAAC8850.020.7842771
TAACACT16050.020.6327464
CTGTCGC23500.020.5246039
GGCTGGA90950.020.3861871
CTGGAGT77950.020.1332843
TTGCTCT37000.020.0405544
Produced by FastQC (version 0.11.5)