FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728555
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.042.7049988
GTCTCGC11850.039.7301831
GTCTTGC24400.033.163611
TCGCTCT12200.031.8637314
CGTTTCG1652.5240133E-630.5405469
CTGTCGC18850.028.642619
GTATAAT8600.028.2276331
CTCGCTC14050.027.1557773
TCTCGCT15850.026.8062882
GTACTAC5200.025.4640871
CGCTCTG15300.024.9371665
GTCCGAT1550.001662358623.7299731
TTGCTCT34850.023.5485824
GTGTTAC12200.023.5160141
GGGTTTC47400.022.5034241
TCTGTCA36950.022.405068
GTATTAC6250.022.3631271
GTTTCAC43550.022.1502863
GTGTAAC7650.022.1169551
GTGTAGT15700.022.022021
Produced by FastQC (version 0.11.5)