FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179668
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13150.045.443148
GTCTCGC12700.041.021841
CTGTCGC16050.038.129399
TCGCTCT15450.035.8810044
TCTCGCT15700.033.019932
GTCTTGC29600.031.045481
CTCGCTC16750.030.520163
CGCTCTG17200.027.6259885
GTGTAAT13450.026.8988741
TTGCTCT38850.025.3881994
GTATAAG10700.025.0209791
GTGTAGG16250.024.935671
GTGTTAG20250.024.2979831
GTGTTAC13500.024.1193221
GTGTAGA16500.024.1193221
GTATTAT10050.023.7593331
GTCTCAC36000.023.7173351
GTATTAG11150.023.3622131
GTTAGCC17100.022.3163453
GGTATAC6500.022.2639881
Produced by FastQC (version 0.11.5)