FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179668
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14550.042.9908561
TCGCTCT17100.032.4159244
TCTGTCG14200.029.4037958
GTATTAA10050.029.2896421
GTCTTGC25550.028.5144161
CTCGCTC18400.027.778213
GTGTTAG19350.027.7627241
TCTCGCT20250.027.7455252
CGCTCTG17450.027.2277835
GTATTAG9000.026.1654151
GTCTCAC34250.025.99831
TTCACCG9200.024.2570295
GGGTTTC48850.022.8980851
GTTTCAC46950.022.8462243
GGTATAC6450.022.8186761
GTGTAAG16050.022.4667971
GTCTTAC12950.022.1623081
GTTAGCC17550.022.1503413
TTGCTCT37650.021.7973464
GTGTTAA11500.021.7571131
Produced by FastQC (version 0.11.5)