FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954011
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12700.040.4514541
TCTGTCG11450.037.730118
TCGCTCT13200.033.271234
CTGTCGC13800.031.8268079
TCTCGCT15500.030.1957652
GTATTAG8950.029.1044221
GTCTTGC22750.028.6246781
CGCTCTG13500.028.2649155
CTCGCTC15150.028.0416623
GTCTTAC9400.027.7111261
GTGTTAG18750.027.3991181
ACCGTGT9500.024.253188
GGGTTTC43850.024.091441
TTCACCG7800.023.9985125
GTATTGA8000.023.5159681
GTTTCAC39950.023.070533
GTGTTAC12000.022.9129961
TTGCTCT33750.022.8256364
GTCTCAC28550.022.8095071
GTATTAT8700.022.4555661
Produced by FastQC (version 0.11.5)