FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954011
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12450.042.0014721
TCGCTCT14100.033.6974074
TCTGTCG13100.031.8728568
CGCTCTG14250.030.311035
CTCGCTC15750.029.7101253
GTCTTAC11100.029.194781
GTCTTGC22700.028.8761751
TCTCGCT17250.028.3920882
GTATTAG10150.025.3967151
GTATAAG7750.024.7085461
GTATAAA10050.024.1837351
TACACTG16850.023.070565
GTCTCAC29550.022.9300941
ATTCCGC2252.8029264E-522.3964826
GAGTGCA67100.022.3154226
GTATTAT8600.022.2664221
GTATAAC5650.022.1603181
ACCGTTG1952.7447368E-422.1503688
GTGTAAG14400.021.992851
GGAGTGC62450.021.9020845
Produced by FastQC (version 0.11.5)