FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817806
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.048.9889768
GTCTCGC11500.037.7603231
CTGTCGC13050.037.516699
GTCTTGC26600.036.731141
GTATTAG9350.033.2842751
TCGCTCT13450.033.1879274
GTATTAA8500.030.6524961
TCTCGCT14350.030.1067582
CGCTCTG14900.029.9582315
CTCGCTC13750.029.8484233
GTATTAT7250.026.9530561
GTGTTAT10600.025.945381
TTGCTCT36400.025.9107864
GTCTCGA24800.025.2567986
ACCGTGT8300.025.156268
TAATACT8350.025.0047344
TCTCGAT9250.024.9068587
GTGTTAC11700.024.743231
GTCTTAC9700.024.6220681
GTCTCAC32000.024.4262081
Produced by FastQC (version 0.11.5)