FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817806
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1452.0545485E-839.7175645
GTCTCGC11250.037.3026771
GTCTTGC25700.035.2362441
TCGCTCT12600.033.137374
TCTCGCT14550.029.204522
TCTGTCG12100.027.9623158
GTATTAG9050.026.032691
CTCGCTC14700.025.9548873
GTATTAA7250.024.3719841
CGCTCTG14800.024.32035
TAATACT7250.023.830544
GTACTAC5700.023.249591
GGGTTTC44750.023.0331131
TTGCTCT35050.023.0033264
GTACAAT8650.022.9808081
CTCGATC10450.022.733088
GTTTCAC42100.022.5710873
GTGTAAC8250.022.3102131
GTGTTAG18250.022.1879651
GTACAGA14300.022.1385961
Produced by FastQC (version 0.11.5)