FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887243
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10750.042.1946378
CTGTCGC13650.034.81269
TCGCTCT14400.034.4982264
GTCTCGC13300.033.190421
GTCTTGC23600.030.3569221
CGCTCTG15800.028.251715
CTCGCTC15300.026.8263283
TCTCGCT15950.026.6360072
GTCTTAC10650.025.141241
GTATTAT9650.024.746921
GTATAGA10400.024.353941
TTGCTCT33700.023.9275514
GTTTCAC39700.023.5792883
GTGTTAG20250.022.1565021
GGGTTTC44900.021.9193211
GTATAAG9400.021.555831
GGTATAC5800.021.2107221
TTCGCTC3758.414645E-921.1222063
GCGCAAC2404.4292414E-521.1067491
GTACGGG2404.4292414E-521.1067491
Produced by FastQC (version 0.11.5)