FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887243
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.037.9782948
GTCTCGC13450.036.6546551
TCGCTCT14050.034.8415074
GTCTTGC24750.032.703491
CTCGCTC15050.029.6564773
TCTCGCT16750.028.8099542
CGCTCTG16300.026.498915
GTGCGAC2309.704254E-725.5940341
CTGTCGC20050.025.4922629
GGGTTTC46300.024.3157141
GTGTTAC12050.023.8151971
GTCTAGC11500.023.6744821
GTTTCAC42900.023.6606253
TTGCTCT36400.023.3374
TCTGTCA34900.023.3086628
GTGTTAG19300.022.494241
TAATACT9400.022.2092844
GTTAGCC15600.022.1503723
GTACTAT7400.021.8759821
GTACTAG5400.021.8023261
Produced by FastQC (version 0.11.5)