FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793664
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13300.040.0538838
GTCTCGC10900.039.164621
TATACGG1406.992086E-735.9943665
TCGCTCT14000.034.4517484
GTCTTAC12200.034.3880421
GTATTAG7950.031.4777871
CTCGCTC13900.031.0742723
GTCTTGC26550.031.0488781
TCTCGCT14800.029.6848472
CGCTCTG15300.029.1719065
TAATACT7150.027.1845554
GTATAAC5450.025.6595781
TACACTG15700.025.2189855
CTGTCGC20100.025.0707049
GGTATAC4900.024.033461
GCGAACC1801.5960401E-423.9962463
GTCTCAC30450.023.6881541
GTGTTAG17450.023.620281
TCTTACT16550.023.4997082
TTGCTCT35900.023.4615084
Produced by FastQC (version 0.11.5)