FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455136
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10300.041.2468538
GTCTCGC10800.039.5192031
TCGCTCT12100.038.0802574
CTGTCGC11900.036.9112639
GTCTTGC23400.032.4603581
CGCTCTG12550.032.1254775
CTCGCTC13400.030.0913453
TCTCGCT13950.029.4211062
GTGTAAT10750.028.9360941
GTATTAA9000.027.3285331
GTGTAAC7700.027.2450221
GTGTTAG17300.025.9254021
GTATTAG7700.024.426571
GTATAGG5700.024.113411
TTGCTCT31100.024.0756634
GTGTTAC9500.023.6057591
GTCTTAC8900.023.5715371
GTGTAAG12850.023.0813181
TACACTG16650.022.9174845
TTACACT12600.022.8557114
Produced by FastQC (version 0.11.5)