FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049335
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC17000.037.5800321
GTATAAC3400.032.0284351
GTGTTAA7950.028.3085691
GTCTCGC8800.028.0491451
TAATACT6100.025.9649684
GTCCTAT6900.025.2514021
TTATACT6050.024.9895764
GTATTAC5300.024.6558511
TCTGTCG8200.024.5832428
GTTTCAC28950.024.3768983
GTGTAAA11350.024.3058681
TCGCTCT10100.024.2355294
GTATTCG1801.5071953E-424.199261
GTATAAT5750.023.9888341
GGGTTTC32250.023.4113791
GTAATAC4353.6379788E-1223.1760673
TTGCTCT26150.023.1261084
GTATAGC4505.456968E-1222.5859781
TCTTGCT28450.022.2747042
CTCGCTC10700.022.2091063
Produced by FastQC (version 0.11.5)