FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049335
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8350.042.2482078
GTCTCGC8900.039.9669571
CGTTTCG1459.1941183E-734.7559139
CTACGCG850.002838380933.879719
GTCTTGC18650.033.863781
CTGTCGC10750.033.485769
GTCTTAC7000.033.185391
TCGCTCT9400.030.6359084
TACCCGT1952.7745045E-729.536165
ATACCCG1952.7745045E-729.536164
TCTCGCT11050.028.0295932
CTCGCTC9850.027.0442353
GTATAAT5450.026.6396481
GTGTTAG12600.026.5022221
GTGTTAC7600.025.7896351
ACCCGTT2551.0161966E-725.4097826
CGCTCTG10550.025.2492165
GGGTTTC31750.024.9217071
TACACCG1450.00127796324.8256515
TAATACT7000.024.6837924
Produced by FastQC (version 0.11.5)