FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982032
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA907.2515395E-540.4958081
GTCTCGC14050.038.3917541
TCGCTCT13650.037.974284
TCTGTCG13850.036.3863078
GTCTTGC25200.033.553671
CTCGCTC15550.033.3343353
TCTCGCT17350.031.142262
CGCTCTG14650.030.9594085
CTGTCGC17850.029.8457989
GTCTCAC30250.026.2653791
GTGTAAA14550.025.549931
GAGTGCA68250.024.57786
GTCTTAC10450.024.413741
GTATAAC4550.024.0304781
GGAGTGC63400.023.9597845
GTACAAC6400.023.9178351
GTGTTAG18800.023.6512741
GTATTAA7100.023.6130491
GTATTAC6350.022.9582521
TCTGTCA34700.022.6144858
Produced by FastQC (version 0.11.5)