FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921718
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12550.044.1700828
GTCTCGC12100.039.7159921
GTCTTGC25500.035.9780161
CTGTCGC16550.035.6695029
TCTCGCT14900.033.8377232
TCGCTCT13900.032.6297954
CTCGCTC15600.030.0108383
GTATTAA9100.029.6051451
CGCTCTG16250.027.0244985
GTATTAC6550.026.6794451
GTCTCAC33650.026.3986431
TTGCTCT34950.024.100574
GTGTTAC10600.024.0419141
GTTTCAC43050.023.0884763
GTGTAGA15600.022.8706421
GGGTTTC46700.022.76371
CTGTTGC28650.022.363859
GTCTAGC12850.022.098781
GTTCTAC9400.021.6888751
CTTGCTC34300.021.4188123
Produced by FastQC (version 0.11.5)