FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921718
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.045.971081
TCTGTCG12550.040.154518
GTCTTGC27850.035.8595661
TCGCTCT14450.035.3734934
CGCTCTG15050.031.0931225
CTGTCGC16950.030.1556519
CTCGCTC15300.029.6444953
TCTCGCT16150.028.550562
GTATTAG9250.026.7945751
TTGCTCT36400.026.3049644
GTATTGG9200.026.1478371
GTACAGG13600.025.1923451
GTGTTAG20400.025.0136761
TAATACT9400.024.5080994
GGCTAGA15650.024.2213651
GGAGTGC68700.023.6831365
GTCTTAC9600.023.5396581
GTGTTAC12400.023.5151621
GCTCTGT66650.023.2234026
GAGTGCA75250.023.1524186
Produced by FastQC (version 0.11.5)