FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662198
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.048.214738
GTCTCGC11500.043.7106821
TCGCTCT12250.041.1382064
CTGTCGC14950.038.524079
CTCGCTC13000.037.1299973
TCTCGCT14200.035.514292
GTCTTGC23500.032.24051
CGCTCTG14300.032.220135
GTCGTAC1203.946516E-430.3546411
GTATTAC6200.028.200441
GTCTTAC9500.024.539331
GTATAGG6850.024.4609661
GTATTAT9050.024.1495481
GGTATAC5150.024.0479471
GTACTAG4850.024.0333651
GTATTAA7200.023.271891
GTGTAAT9600.022.765981
TTGCTCT32050.022.6869814
GTATAAG8050.022.6245751
TTACGCA1600.002266875722.4974564
Produced by FastQC (version 0.11.5)