FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680307
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.036.788698
GTCTCGC11950.036.6302381
TCGCTCT11900.032.064714
GTCTTGC22650.031.5656131
TTCACCG7950.029.8844475
TCTCGCT15650.029.0006852
GTACTAT6100.028.703691
GTTTCAC40000.028.2577823
CCGTGTT7950.028.072229
CTGTCGC15200.027.47059
CGCTCTG13400.027.4008335
CTCGCTC14550.027.2143573
GTATTAT8750.026.6807631
GGGTTTC45000.026.4261
TTACACT12900.026.23054
GTCTCAC31850.025.8836381
GTCTTAC10550.024.8946691
TAACACT13250.023.9075584
GTATTAG7950.023.8595681
TCACCGT10600.023.7717176
Produced by FastQC (version 0.11.5)