FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412338
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10050.053.725218
GTCTCGC10700.042.2215651
TCGCTCT12450.038.7425654
CTGTCGC14650.037.347279
CTCGCTC12050.034.671053
TCTCGCT12950.034.4871252
GTCTTGC23200.032.978261
CGCTCTG13400.031.1606225
GTCTCAC28500.028.6351591
TACCCGT2105.3078475E-727.4254425
GTAATAC6300.026.297433
CTTGCTC29100.025.4958953
TTGCTCT31750.025.3955274
GTATTAG8700.025.1262931
GTTTCAC35250.024.1128713
GTCTTAC10000.023.31721
GTATTGC7600.023.0103951
GTATTAT8650.022.7443791
GTATATA11000.022.5222971
TACACGG2954.150952E-721.9635965
Produced by FastQC (version 0.11.5)