FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709963
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.049.5375378
CTGTCGC14550.038.595029
TCGCTCT12350.034.3947944
GTCTTGC26950.033.1106831
GTCTCGC12200.032.111141
GTATTAG8100.028.6606961
GGTATAC5450.026.6228941
GTATTAA8850.026.2318251
TCTCGCT15150.026.1458032
CTCGCTC15600.025.39163
GTATAGA7850.024.9526061
GTATTAT8800.024.7320651
TAATACT7600.024.630174
GCGTAGT3301.7626007E-924.1824631
CGCTCTG14950.023.5964435
GTATTAC6250.023.2151661
CTAGTAC5000.023.0463373
TTGCTCT35950.022.6301384
GTTCCGA2303.128746E-522.0796411
GTGTTAC11850.022.0397151
Produced by FastQC (version 0.11.5)