FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709963
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.040.1798868
GTCTCGC12100.038.664631
TCGCTCT13200.031.086144
GTCTTAC10400.030.9270571
CTGTCGC16900.030.6698519
GTCTTGC24650.029.0621971
TCTCGCT15800.028.7071062
CGCTCTG14400.028.4956285
GTGTTAC11300.027.1700291
GCGTTAC1657.964234E-526.5819341
GTATAAT8550.025.6492331
CTCGCTC16000.025.648433
GTCTCAC33100.025.397391
GGTATAC5150.024.1302031
GTATTAC7000.024.0186771
GTGTAAA17500.023.8098161
TTGCTCT34850.022.7224834
GTGTAAG12900.022.1000981
GTCTAGC12300.021.9895251
GTTTCAC39850.021.8606473
Produced by FastQC (version 0.11.5)