FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351717
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC24650.035.0369451
TCTGTCG9600.033.7439428
GTCTCGC9400.032.4278141
GTATTAC7400.032.365211
CTGTCGC12000.029.3947269
TCGCTCT11750.028.799124
GTATTAG9250.026.676781
GTGTTAC11800.025.217271
TTGCTCT34900.024.9619884
GTGTTAG16850.024.5511131
TCGGAAC2101.639577E-524.0043723
TCTCGCT12300.024.004372
CTATACT7300.023.6705114
GTATAAT7400.023.5383361
CTCGCTC12250.023.5144863
CTTGCTC33000.023.3497073
CGCTCTG14200.023.319765
GACCGTA1550.001885495323.221647
GACCGAT1600.002162710822.6801681
GTCCGGG5500.022.4327471
Produced by FastQC (version 0.11.5)