FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530101
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10600.041.3948971
TCTGTCG10750.035.4930538
TCGCTCT11150.032.2840654
GTACGGC1753.5310914E-629.2523961
GTATTAG9000.027.6272621
CTCGCTC13800.027.131193
TCTCGCT14850.026.6684652
CTGTCGC14250.026.2692229
CGCTCTG13650.025.8432985
GTATTGG9400.024.8956571
AGTACCG1450.001278288224.8248425
GTATAAT8900.024.6508961
TATACCG1500.001557374323.9973495
GTGTTAG19200.023.9961051
GTCTTGC23300.023.8538881
GGTATAC6150.023.7824361
GTCTTAC11850.023.4512881
GTACTAC5000.023.4019171
GTATTGA9350.022.6823391
GTGTTAC11350.022.5514071
Produced by FastQC (version 0.11.5)