FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482499
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.036.8653768
GTCTCGC11150.034.4919661
GTCTTGC25850.033.1236651
TCGCTCT12100.032.130524
CTGTCGC13700.031.003779
GTACGCC1402.8800147E-530.8592283
GTATTAT7750.029.961611
TAACACG5600.026.998564
CTCGTCG1358.42522E-426.6636359
TCTCGCT13050.026.4845472
CTCGCTC13650.026.3754083
CGCTCTG14500.026.3142495
TTGCTCT33550.025.7512384
GTGTAAA14800.024.024331
GTATAGT8850.023.7777981
GTGTTAC10750.023.6252541
GTACTAG4950.023.4547961
GTATTAG8800.023.0883161
CTTGCTC32800.022.830813
GTCTCAC32000.022.6760251
Produced by FastQC (version 0.11.5)