FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482499
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.040.594318
GTCTCGC12000.035.9490361
TCGCTCT12450.035.272224
GTCTTGC23650.030.6069831
CTGTCGC16150.029.865839
CTCGCTC13500.029.3351763
GTATAAT8700.027.7339131
CGCTCTG14300.027.1850415
GTGTTAC12250.026.262321
TCTCGCT16150.025.4143852
GTATTGG10550.024.2567181
GTATAAG8450.024.2280141
TTACACT13750.024.0839354
GTGTTAG17700.023.546051
GTATTAA8850.023.1329611
GTACTAT6200.022.4067251
TCGATCT10300.021.66699
GTATAAC5450.021.4654481
GTGTAAT13650.021.4261341
GAGTACG2053.4645412E-421.4000031
Produced by FastQC (version 0.11.5)