FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471262
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9550.031.661598
GTCTTGC19500.030.1657731
GTCTCGC8850.029.5408691
GTATTAA6800.027.7669721
GTATAAG8450.026.6421361
CTGTCGC13050.025.376649
GTCTCAC25850.024.7221471
TTGCTCT29050.022.8024354
GTGTTAG17500.021.9938811
TCGCTCT11200.021.8574964
CCCGCGT2053.846789E-421.0709538
CCGACCG1750.003812284720.5692639
ACCCGCG2506.311137E-520.1578777
ACTGTCG3301.2187484E-619.6342988
GTCATAC5555.456968E-1219.627381
GGATAAA11150.019.5393641
AGCGCCG2951.0170143E-519.5233695
TAACACT11500.019.4090084
TCTCGCT11600.019.2463632
GGTATAC4202.8074282E-819.0198651
Produced by FastQC (version 0.11.5)