FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366408
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.038.593048
CTGTCGC11650.034.605059
GTCTCGC10950.033.786991
TCGCTCT11300.031.2192554
GTCTTGC22700.030.9984051
CTCGCTC11150.028.4185583
TAATACT9250.028.019844
TCTCGCT12000.027.605832
GTATTAC6700.025.9854281
GGTCGAT1701.0277567E-425.603291
GTGTAAC7850.024.9509771
TAGTACT5900.024.405234
GTGTAAT11850.023.8747981
GTCTAAT7050.023.6663991
CGCTCTG12550.023.5189275
GTATTAG8850.022.9513511
GTGTAAA15900.022.3559111
GTTAGCC12000.022.2046893
GTATAAT8500.022.1895181
GTATTAA8850.022.131661
Produced by FastQC (version 0.11.5)