FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539719
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12200.041.621981
TCTGTCG11300.038.864888
GTCTTGC25350.033.4805341
TCGCTCT12950.033.3570334
CTGTCGC13450.032.6522759
TCTCGCT15500.029.731312
CGCTCTG14000.028.2839835
CTCGCTC15450.027.0311973
TTGCTCT35300.026.9218644
GTATAAT8100.026.8670981
GTATTAC6000.026.5984271
GTATTAA8800.025.5542741
CTTGCTC34500.024.2105483
GTATTGC8300.023.5977271
TATACTG9250.023.3499225
GTATTGG9800.022.9466931
GTCTTAC11300.022.4685021
GTGTAAC10050.022.3758811
GGGTTTC44600.022.2828241
TCTTGCT41350.021.0705362
Produced by FastQC (version 0.11.5)