FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539719
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11400.039.1536068
GTCTTGC24850.037.369581
GTCTCGC10300.036.915351
TCGCTCT13300.035.7261124
TGTACCG2206.730261E-1032.7243355
CTGTCGC14650.030.4658559
GTCTTAC10500.028.5519311
GTATTAG9500.028.4786241
CTCGCTC14050.027.6728343
CGCTCTG15100.027.1763675
GTGTAAT12700.027.060451
TCTCGCT14650.027.0320152
ACCGTGT9050.026.2512688
TTCACCG7550.024.7924755
CTTGCTC35000.023.8630643
GTATTAA8300.023.786281
GTGTTAC12100.023.567861
GTGTAAA19550.022.8151841
GTACAAT9300.022.801111
CCGTGTT8600.022.6008429
Produced by FastQC (version 0.11.5)