FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572927
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11450.037.7250568
CTGTCGC13350.033.4344759
GTCTCGC11700.031.5935541
TCGCTCT12900.030.1391034
GTCTTGC26050.028.9360871
GTACTAG5550.027.424611
TCTCGCT14000.026.2327842
CTCGCTC14800.025.3013613
GTATTAG9850.025.0182461
GTGTAAC9100.024.6907621
TTACGCT2051.3625771E-524.5850144
TTCACCG7350.024.486595
CGCTCTG15300.024.4673845
GTACCGG2701.6555168E-724.1597771
TACCGGA1500.001554924324.0038572
TTGCTCT34850.023.7586254
GTCTTAC10850.023.3804281
GTCTCAC30850.023.2591671
GTTTCAC40800.022.9448623
GTGTTAC12650.022.9183641
Produced by FastQC (version 0.11.5)