FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460991
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.037.5591668
GTCTCGC10900.032.605541
CTGTCGC13700.029.4259139
TACACCG1753.9916795E-628.7953595
GTCGAAC1050.007694683527.630741
GTATCGC1050.007694683527.630741
GTCTTGC24350.026.8080621
TCGCTCT11200.025.7111874
TCTCGCT12650.025.6163272
CGTTTCG1801.5960154E-423.9961329
GTATTAG10100.023.6981491
GTATAAC4353.6379788E-1223.3432141
GGGTTTC44000.023.0779481
TAGTACT6100.022.4235274
GTTTCAC42750.022.0663283
GTATAAT8950.021.880771
GTGTTAC12000.021.7592091
TATTAGG9300.021.6805342
GTGTAAA15450.021.5949021
GTATAGA7450.021.418461
Produced by FastQC (version 0.11.5)