FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460991
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11150.041.2855221
TCTGTCG11900.039.930198
TCGCTCT12400.035.4192244
GTCTTGC22950.033.4301761
TCTCGCT14350.031.6115682
CTGTCGC15400.029.4508469
CTCGCTC14750.029.2898123
CGCTCTG14850.028.1211095
GTATAAT8150.026.8964981
GTATTGG9150.025.5541231
GTATTAG9200.025.4152411
GTACTAA5550.025.014551
GTTAACG1500.001555841224.0013753
GTCTCAC30300.023.3916681
TTGCTCT36500.022.8821074
GTGTTAG17950.022.7958431
TAATACT8950.022.5250574
GCTCTGT61700.022.522716
GTCGTAA1650.002486486222.1420651
GTGTTAC11750.021.7651791
Produced by FastQC (version 0.11.5)