FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676484
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.039.383018
TCGCTCT13150.035.0403984
CTGTCGC14100.033.7007569
GTCTCGC13300.032.482911
GTATAAC5400.029.3348791
CTCGCTC15300.028.2346733
GCGTAAC1306.751522E-427.6937661
TCTCGCT15450.027.4965952
GTCTTGC26900.026.4995371
GTCTTAC11750.026.3503251
GTGTAGA15150.026.139991
CGCTCTG16800.025.2846855
GTGTAAT13100.025.2837771
GTGTTAC12550.024.6706241
CTTGTCG2351.4150646E-624.509649
GTATTAA8650.024.141
GTCGACC1550.001884042623.2248618
GTATAAG10250.023.181711
GTGTTAG22250.022.0056531
GGGTTTC48900.021.7925591
Produced by FastQC (version 0.11.5)