FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676484
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11200.038.575551
TCTGTCG10600.035.9951028
GTACCGA1501.1969078E-633.603591
CTGTCGC13350.032.89449
TCGCTCT13500.030.3964314
GTATTAT9250.029.5815371
TCTCGCT14500.029.2942092
GTCTTGC26750.028.533891
CTCGCTC15050.028.2225993
TAGTACG1306.7582406E-427.6890544
GTCTTAC12550.025.819491
CGCTCTG15100.025.745325
GTATTAC7800.024.925741
GTATTAG10700.024.8998571
GTCTCAC32150.023.9652351
GTATAGT8200.023.709851
TACACTG18550.023.6737695
GGGTACG2452.0309162E-623.5127161
GTACAAG9400.022.9811781
GTGTAAC10050.022.9278221
Produced by FastQC (version 0.11.5)