FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338212
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10750.040.188951
TCTGTCG9400.037.5306138
TCGCTCT12000.032.998854
CTGTCGC12600.027.9990279
TCTCGCT13900.027.9732442
GGTATCG1555.766384E-527.872981
CGCTCTG13950.027.3538885
CTCGCTC13950.027.3538883
GTATAGG6750.026.6685891
TTCACCG7350.026.4480615
GTATTCG1400.0010406225.7161431
GTATAGA8000.025.2018181
GTCTTAC10650.025.015891
CCGTGTT7950.024.451989
GGGTTTC40900.024.295151
GTTTCAC35850.023.8987523
TAACACG5750.023.790484
GTATTAC6400.023.6267051
GTCTCAC30500.023.6082611
TCACCGT8800.022.9082976
Produced by FastQC (version 0.11.5)