FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844929
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13000.039.888691
TCTGTCG13200.037.6342288
TCGCTCT14700.035.7530754
GTCTTGC26350.034.1656761
CTGTCGC15950.031.5969569
CTCGCTC16350.029.9437853
GTATTAC8350.029.3260141
TCTCGCT16900.028.545272
CGCTCTG17050.026.6030655
GTATTAG11800.026.2450281
GTATTAT9750.025.1150991
GTCTCAC31500.024.9216371
GTATACG1751.3143297E-424.6929971
GGGTTTC51150.024.4998951
GTTTCAC48200.023.6007393
GTATTGG11300.023.5821761
GTACGGC2202.3479006E-522.915851
ACCGTGT9850.022.6586158
GTGTTAG20050.022.6301161
GTACTAC4505.456968E-1222.4066091
Produced by FastQC (version 0.11.5)