FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393781
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTTCGATATCTCGTATGCCGT25090.10481326403710281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.035.998418
GTCTCGC10950.030.2477651
GTATTAT4300.030.1407181
GTCTTGC23650.028.9229131
CTAACCG1000.006286409728.7987254
CTGTCGC12900.027.905749
TCGCTCT12300.027.5109774
GAACGAT3057.730705E-1025.9660666
GTATTAC3501.4188117E-1024.6866861
TCTCGCT12600.024.5723952
GTCTCAC27100.024.4437661
GTACTAA4151.8189894E-1224.2901121
TTGCTCT29450.023.224784
CTCGCTC12800.022.4994753
TACCGTG2654.0190935E-621.7348887
CGTCAGA4359.276846E-1121.5180871
GTGTTAG14400.021.5008451
CGCTCTG13750.021.4681425
GTCTAGC8150.021.2032871
CTTGCTC29650.020.3975013
Produced by FastQC (version 0.11.5)