FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9650.041.7806788
GTCTCGC10900.037.652071
TCGCTCT10500.033.599294
CTGTCGC12850.031.376159
GTCTTGC24850.031.2923241
TCTCGCT12500.029.3765372
GTATTAT7700.028.0524621
TCCGTCG1050.00797704927.4274528
CTCGCTC12750.027.105313
GTGTAAA15700.024.7647861
GTATTAG10000.024.4804481
TTGCTCT33100.024.3620244
TTCACCG7750.024.1538525
GGTTACG1801.594092E-424.000441
CGCTCTG13600.023.2931675
GTCTAAC4950.023.2731551
GTGTAAC8000.022.5004141
GTATTAA7950.021.7362481
TAATACT7650.021.6466034
GTTAGCC15350.021.5760923
Produced by FastQC (version 0.11.5)