FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534092
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.045.7839978
GTCTCGC12300.038.05291
CTGTCGC16150.035.6603439
GTCTTGC25950.034.9633181
TCGCTCT13150.032.3006634
TCTCGCT14550.029.1944242
CTCGCTC14800.028.6995743
GTGTTAC10500.026.7457491
GTACTAG5550.025.9487531
CGCTCTG14500.024.824885
TTGCTCT36000.024.3973434
GTATTAT7750.024.1574521
GTATTAG8500.023.7202111
TAATACT8450.023.0034124
TACACTG18200.022.1514345
GTATATA11450.022.0111141
GTCTTAC11500.021.9154131
TAGTACT6000.021.5976494
GTGTATA16550.021.3195271
GTATTAC6100.021.2482011
Produced by FastQC (version 0.11.5)