FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666641
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9500.045.4717258
CTGTCGC12150.035.5540249
TCGCTCT12650.033.580214
GTCTCGC11050.033.2324521
GTATAGG7150.029.2042751
GTCTTGC25900.028.9126631
TCTCGCT13550.027.1010062
CGCTCTG14800.026.269635
GTGTAAC9500.025.0117931
TTCACCG6300.023.9994145
CTCGCTC15500.023.6897473
GTATAGA8550.023.5801431
TAATACT7400.023.3507824
GTGTTAC12650.023.3371521
GTCTTAC10800.023.3345171
GTATTGA10700.022.8796631
GTATTAA7250.022.8425371
GTATTAG10450.022.7379931
GTTTCAC41850.022.7091243
TCTGTCA34500.022.5381588
Produced by FastQC (version 0.11.5)