FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666641
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.038.9310958
GTCTCGC12000.037.2040441
GTCTTGC23450.035.005941
TCGCTCT14800.033.5649224
CTGTCGC15250.029.7408039
CGCTCTG16600.028.6247865
TCTCGCT16650.028.5409682
CTCGCTC17950.026.8740483
GTATTAT8600.026.793611
GTCCTAC8300.025.1593631
TTGCTCT33950.024.8110054
GTCTTAC10750.023.444411
GTGTAAT11850.023.091121
GAGTGCA67600.022.5772556
GGTATAC6400.022.5024491
CGTAGTA1600.002265190522.5003382
GTACTAT7250.021.850651
GGAGTGC62100.021.7957945
GGGTTTC47600.021.7838841
CTGTTGC28900.021.672229
Produced by FastQC (version 0.11.5)