FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235221
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.051.9078188
GTCTCGC15050.039.9833031
CTGTCGC18850.037.432979
GTATTAC7850.035.0954671
TCGCTCT16650.035.0275964
GTCTTGC32250.032.8216361
TAATACT9500.031.8320944
CTCGCTC18100.031.0444433
TCTCGCT18650.030.5114152
CGCTCTG19600.028.2861715
GTGTTAG22500.028.0332741
GTATTAT10100.025.8415281
GTATTAG10700.025.7476081
GTAGCGC2001.1205053E-525.213663
GTATAAC5300.024.6225871
GTTTCAC49200.024.3058513
GTATAGT9550.024.293141
GGGTTTC54150.024.099671
GTGTATA19550.023.3631211
GTGTTAC14950.023.2775441
Produced by FastQC (version 0.11.5)