FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087853
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC27900.032.745391
GTCTCGC12500.030.404491
TCTGTCG13050.028.6867688
GTATTAG12500.028.0656831
TCGCTCT15200.026.999034
GCGCTAA2507.251765E-826.3115771
GGGTACG3102.2231688E-823.5766811
GTATAAC6000.023.1444421
CGCTCTG17350.022.8226495
CTCGCTC15600.022.6145743
GTGTAAG17350.022.3265061
GTCTCAC38900.022.1705651
TCTCGCT16900.022.1541292
GTATAGG9750.021.7389091
GTGTTAG22900.021.7029041
GTATTAT11150.021.631341
GTAATAC7000.021.5992263
GTATTAC7500.021.4390621
CTGTCGC19550.021.3584639
GAGTGCA78850.021.2740446
Produced by FastQC (version 0.11.5)